FIG. 2.
ASC viability and growth factor production. ASCs were fed at day 0 and maintained in 0.2%, 2%, or 20% O2 for 6 days. (A) Live/Dead staining was used to assess viability at day 6. The number of viable (green) cells appeared similar in all groups. (B) The total DNA content normalized to day 0 was also similar in all groups. (C, D) VEGF and FGF-2 production was assessed in all groups using ELISA after 48 h in serum-free medium and normalized to DNA content. Scale bar = 500 μm. Significance indicated versus normoxia (20% O2). FGF-2, fibroblast growth factor-2; VEGF, vascular endothelial growth factor. Significance is indicated as *p < 0.05 or **p < 0.01. Color images available online at www.liebertpub.com/tea