Skip to main content
. 2015 Nov 25;22(1-2):161–169. doi: 10.1089/ten.tea.2015.0421

FIG. 5.

FIG. 5.

Comparison of ASC metabolic activity and gene expression in immediate or delayed hypoxia. Glucose consumption and lactate production increased in 0.2% and 2% O2 relative to 20% O2 after 6 days of immediate hypoxia (de novo vascular assembly) (A) and delayed hypoxia (6 days of preculture in normoxia) (B). By day 12, continued normoxic culture resulted in reduced glucose and lactate metabolism versus day 6 (dotted lines), whereas hypoxic conditions increased glucose consumption. (C) mRNA expression of VEGF-A was substantially upregulated in hypoxia, with highest expression in 0.2% O2. VEGFR-2 expression was reduced in 2% O2, but unchanged in 0.2% O2 relative to normoxia. (D) Changes in VEGF-A and VEGFR-2 expression were even greater following delayed-onset hypoxia. Significance indicated versus normoxia (20% O2) as *p < 0.05, **p < 0.01, or ***p < 0.001. VEGFR-2, VEGF receptor-2.