Fig. S2.

(A) siVNUT does not affect calcium dynamics. Cells nucleofected with siVNUT or scramble oligonucleotides tagged with Cy3 were loaded with the Fluo-4 calcium indicator. We only used red-tagged cells for the analysis. (Scale bar: 20 μm.) (B) The cells were stimulated with four successive DMPP pulses (see above) and the fluorescence was recorded continuously. Note the progressive reduction in intracellular calcium. (C) Pooled data represented as the mean ± SE from control (n = 14) and siVNUT1 (n = 11) nucleofected cells.