Fig. S5.

Noncentrosomal Mps1 does not accelerate centriole reduplication. (A) Representative images of U2OS cells transfected with the indicated constructs after a 24-h S-phase arrest. At 24 h after transfection, S-phase arrest was achieved by adding HU for 24 h; 48 h after transfection was considered to be t = 0 of S-phase arrest and was followed by addition of fresh HU for a further 24 h. DNA (blue), GFP (green), centrioles (Cetn2, red), centrosomes (γ-tub, magenta). (Scale bar, 5 µm.) (B) Percentage of GFP⁺ cells with indicated number of Cetn2 foci, presented as the mean ± SD of n = 3 independent experiments of n = 100 cells for each experiment.