Table S2.

Summary of characteristics of mAbs

											Rearrangement
		Binding to ZIKV†		Neutralization—FRNT50 titer‡, μg/mL					Ig isotype		Heavy chain			Light chain
Patient*	Ab	Lysate	Whole virus	ZIKV	DENV1	DENV2	DENV3	DENV4	Heavy chain§	Light chain	V	J	Mutations	V	J	Mutations
31	3H04	+	+	—	1.3	0.2	0.4	0.4	IgG1	Igκ	4–30	4	16	1–12	1	13
	3F03	+	+	—	—	1.2	—	—	IgG1	Igλ	3–43	4	22	7–43	2	19
	3H02	+	+	—	—	1.6	—	1.8	IgG1	Igκ	3–30	4	22	3–11	2	9
	3G06	+	+	—	7.5	0.4	—	2.5	IgG1	Igλ	3–30	4	18	2–23	2	19
	3G02	+	+	—	—	0.2	1.8	1.2	IgG1	Igκ	3–13	4	15	2–30	4	6
	3E03	+	+	—	—	1.0	6.2	4.5	IgG1	Igλ	3–43	4	19	7–43	2	14
	3G05	+	+	—	—	0.4	0.5	3.0	IgG1	Igκ	3–23	4	25	3–20	3	16
	3F02	+	+	—	—	0.2	0.9	0.5	IgG1	Igκ	3–30	4	29	2–30	2	2
	3F01	+	+	5.0	—	0.3	—	—	IgG1	Igκ	3–30	4	23	2–30	4	11
	3D02	+	+	—	—	3.5	—	—	IgG1	Igκ	3–23	4	23	3–20	3	14
	3C04	+	+	—	—	0.6	—	3.1	IgG1	Igκ	1–2	4	16	3–20	4	11
	3D03	+	+	2.3	—	1.9	4.1	—	IgG1	Igκ	4–4	3	5	1–5	2	7
32	2D03	—	+	1.1	0.8	7.0	4.3	—	IgG1	Igκ	3–21	3	12	1–5	2	20
	2E04	—	+	1.6	1.0	0.7	4.0	—	IgG1	Igκ	1–46	6	17	2–28	2	5
33	3G04	+	+	—	3.1	0.5	—	2.9	IgG1	Igλ	3–21	4	14	1–44	3	6
	3F05	+	+	4.3	—	0.2	—	1.2	IgG1	Igκ	3–11	4	10	1–33	1	10
	3D02	+	+	—	2.1	0.6	2.2	0.5	IgG1	Igκ	3–15	4	12	3–20	2	8
	3A06	—	+	0.03	0.2	0.5	1.4	—	IgG1	Igλ	4–61	5	35	1–44	3	18
39	3A04	+	+	—	7.7	0.3	4.7	1.6	IgG1	Igλ	4–39	4	24	2–14	2	12
	3D06	+	+	—	—	1.0	—	7.2	IgG1	Igλ	7–4	4	16	7–43	3	32
	3G02	+	+	—	2.9	1.9	6.3	—	IgG1	Igλ	3–30	4	24	1–9	4	21
	3B02	+	+	—	—	1.2	—	—	IgG1	Igλ	3–21	4	7	1–47	3	12
	3D01	+	+	—	—	1.0	0.5	1.1	IgG1	Igλ	3–21	4	20	1–47	3	13
	3A02	+	+	—	3.6	0.7	3.5	2.1	IgG1	Igλ	3–7	3	31	2–14	2	23
	3C01	+	+	—	4.2	1.4	4.4	—	IgG1	Igλ	1–69	6	24	1–51	2	8
	3D02	—	+	5.2	0.8	0.6	—	—	IgG1	Igλ	4–59	5	9	1–44	3	12

^*All patients were infected with DENV2.

^†Maximum mAb concentration tested = 10 μg/mL.

^‡FRNT50 values below 8 μg/mL are shown.

^§All mAbs were cloned into heavy chain expression vectors containing the IgG1 constant region.