Fig. S4.
Bacterial expression and interaction analyzes of Gpr161-CT and RIα. Following bacterial coexpression of hexa-histidin (his6) and s-tag tagged CT378–528 (wild-type and L465P) and untagged RIα (schematics are shown), we purified complexes using Ni-NTA resin. Subsequent to imidazol elutions, CT378–528-bound complexes have been subjected to cAMP precipitations (±excess of cAMP; 5 mM). Asterisks (*) indicate specific Coomassie brilliant blue-stained bands. IB (with GPR161 and RI antibodies) has been performed to identify protein bands.