Table S1.
Off target analysis of Mkx−/− rats
| Site name | Sequence | Chromosome | Primers for sequence | |
| Target | ACTCTTGGCTCTAGG | Ch17 | Forward 5′–3′ | Reverse 5′–3′ |
| Off 1 | ACTCTTGGCTCTAGG | Ch 6 | GTAGCATTCAGTAGCTTCCCAGTTCAGAC | CTGCCCTTCGACTTGACTTTACTC |
| Off 2 | GCTCTTGGCTCTAGG | Ch 5 | CTCACTTTGGAGATCTGGAAGAAATGTTTGC | CTTTCTTGACATTCTTTCTATTACCAGGCAC |
| Off 3 | CCTCTTGGCTCTAGG | Ch 6 | GAAGTCATTCCCACATGTCCACATTATCATAG | CAGTAACAGACACAGTGGAAGAAAATCAGTAC |
| Off 4 | GCTCTTGGCTCTAGG | Ch 6 | GAGAGATCTTTGGACTGTCAAAACTACTAC | CTGAAATCCTGCCAGGTAAATGGTG |
| Off 5 | TCTCTTGGCTCTAGG | Ch 7 | GGACAAGCCTAAGCAGCTTACTAGAAG | GCAGAATTGGATTTGGCTTCTGGG |
| Off 6 | CCTCTTGGCTCTAGG | Ch 8 | GGTACCACATGGTCCAGAGGCAG | CTCCAGTGGCTCTTTGGAGCC |
| Off 7 | CCTCTTGGCTCTAGG | Ch 8 | GCTCTTCATTCCTGCTTTGGTATCTG | GCCACCTTATCATGTCTTCCTAAAACAGA |
| Off 8 | ACTCTTGGCTCTAGG | Ch 9 | CGTGCTTCTAGTCTGTGAATTATTCGC | GTCAATGAAGGGGCTTCTGTATTTGATG |
| Off 9 | TCTCTTGGCTCTAGG | Ch 9 | GACCTTCATCCCCAATGTAACAGG | GCATTGGCTCATGGTTCTGGAG |
| Off 10 | ACTCTTGGCTCTAGG | Ch 9 | CTGAGAAAAGGCTTTAACGAGTCCATG | GAGGGTCCTCCTAAAATGCCATTATAGT |
| Off 11 | ACCCTTGGCTCTAGG | Ch14 | CCCTATCATCATTCTCCTCGAGGTCTAG | GGAGACACATGGGGCATTTTCCTC |
Off indicates off-target candidate sites. Mismatches from the target are in bold and underlined.