Fig. 2.

Borg proteins colocalize with septins. (A) Caco-2 cells were transfected with SEPT6-tomato and Borg1-, Borg2-, and Borg3-GFP. Cells were treated with CDT (200 ng/mL CDTa and 400 ng/mL CDTb) for 90 min. Borg proteins colocalize with septins. (B) Caco-2 cells were transfected with SEPT6-tomato and Borg2-GFP. Cells were treated as in A. Borg2 colocalizes with SEPT6 at the base of protrusions. (Scale bar, 3 μm.) (C) Caco-2 cells were transfected with Borg3-GFP and treated with CDT as in A. Cells were stained for SEPT2 and tubulin. (Insets) Septin colocalization with microtubles and Borg in control cells. In CDT-treated cells, Insets show chevron-like structures at the protrusion base and colocalization of septins and Borg. (Scale bars, 10 µm.)