Fig. S5.

Formation of microtubule-based protrusions with septin base in primary colonocytes. Indirect immunofluorescence of primary mouse colonocytes for SEPT7 (green) and α-tubulin (red) after 24–36 h in vitro. Cells were treated with 20 ng/mL CDTa and 40 ng/mL CDTb for 1.5 h. Control cells show a similar septin organization as cell lines (first line of images). After CDT treatment, microtubule-based protrusions are formed with septins at their base (gallery starting the second line of images; septin accumulations are marked by white arrows). Magnifications of Insets are below or within the images. (Scale bars, 5 µm.)