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. 2016 Jun 28;113(28):E4015–E4024. doi: 10.1073/pnas.1608795113

Fig. S3.

Fig. S3.

Glycosylation sites and concentration of Htm1p–Pdi1p are not the major limiting factors for demannosylation rates. (A) The change in Man7 abundance relative to the sum of Man7 and Man8 on CPY* after 72-h incubation with Htm1p–Pdi1p. Shown are the mean values ± one SD from an experiment performed in triplicate. (B) Htm1p–Pdi1p generated similar levels of Man7 on CPY* and CPY* variants with either the fourth (CPY*1110) or the first three (CPY*0001) glycosylation sites eliminated. Shown are the mean ± one SD from an experiment performed in triplicate. (C) Increasing the concentration of Htm1p–Pdi1p from 0.1 µM to 0.4 µM did not proportionally increase its Man8-to-Man7 mannosidase activity against CPY* after 20-h incubation. Shown are the mean values ± one SD from an experiment performed in triplicate.