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. 2016 Jun 28;113(28):E4015–E4024. doi: 10.1073/pnas.1608795113

Fig. S9.

Fig. S9.

Folding states of RNase B variants. (A) Each RNase B variant (10 µM) was treated with 50 ng of trypsin in a total volume of 30 µL at room temperature. At the indicated time points, 10-µL samples were collected and mixed with PMSF to stop proteolysis for subsequent SDS/PAGE analysis. (B) Each RNase B variant (10 µM) was treated with 1 unit of PNGase F in a total volume of 30 µL at 37 °C. At the indicated time points, 10-µL samples were collected and mixed with 1× SDS/PAGE sample buffer to stop deglycosylation for subsequent SDS/PAGE analysis. (C) Size distribution of Scr-RB before (control) and after (Overnight) overnight incubation with Htm1p–Pdi1p at 30 °C, as analyzed by Superdex 200 size-exclusion chromatography. (D) Size distribution of Carb-RB before (control) and after (Overnight) overnight incubation with Htm1p–Pdi1p at 30 °C, as analyzed by Superdex 200 size-exclusion chromatography.