Fig. 2.

XerC_Ng and XerD_Ng bind to dif_Ng and dif_GGI. EMSA experiment showing the interaction between an increasing concentration of XerC_Ng (0.4 and 0.6 μM) and XerD_Ng (1.4 and 1.8 μM) and a 28-bp DNA fragment containing either dif_Ng (A) or dif_GGI (C). The color code used is the same as in Fig. 1. Unbound DNA (dif), complexes with one recombinase bound (dif–Xer), and complexes with two recombinases bound (dif–Xer²) are represented. In C, substituted positions in dif_GGI are represented as lowercase characters and highlighted by stars. (B and D) Titration experiment of dif_Ng (B) or dif_GGI (D) by XerD_Ng. The experiment was done in presence (underlined with green) or in absence (underlined with purple) of XerC_Ng (see also Fig. S1E).