Fig. 6.

FtsK stoppage on XerCD/dif complexes. (A) Schematic of the translocation assay using a color code as in Fig. 1. The dotted line represents FtsK translocation, which releases the DNA from the bead (biotine/streptavidin link breakage), allowing its recovery in the bead-free supernatant after bead pull down (Methods). (B) Each gel represents the analysis of free DNA obtained after incubation of the substrate (DNA containing dif_Ng or dif_GGI) with XerCD_Ng, t-FtsKαβγ_Ec or both. The “total DNA” control was obtained by heat denaturation of the different DNA-bead complexes. The “% protection” represents the difference of free DNA obtained after t-FtsKαβγ_Ec incubation in absence and presence of XerCD_Ng (mean of at least three independent experiments with SDs).