Table S1.
Summary of VDJH joins analysis from HTGTS-Rep-seq libraries of C57BL/6 mice
| Cell type | Locus | Input DNA | Coding end primer | Exp.* | No. of V(D)J junctions, including duplicates | Correlation between % productive V(D)J subsets† | No. of unique CDR3 junctions | Correlation between % productive V(D)J subsets† |
| Pro-B | IgH | 2 μg | JH4 | Mouse 1 | 8,299 | vs. M2: 0.88 | 1,639 | vs. total: 0.97 |
| Mouse 2 | 25,957 | vs. M3: 0.98 | 9,133 | vs. total: 0.99 | ||||
| Mouse 3 | 22,613 | vs. M1: 0.85 | 8,894 | vs. total: 0.99 | ||||
| Splenic B | IgH | 2 μg | JH4 | Mouse 1 | 45,857 | vs. M2: 0.99 | 20,583 | vs. total: 0.97 |
| Mouse 2 | 57,477 | vs. M3: 0.97 | 38,091 | vs. total: 0.99 | ||||
| Mouse 3 | 16,504 | vs. M1: 0.96 | 7,053 | vs. total: 0.97 | ||||
| Splenic B | IgH | 2 μg | JH1 | Mouse 1 | 54,172 | vs. M2: 0.98 | N.D. | N.D. |
| Mouse 2 | 77,670 | vs. M3: 0.98 | N.D. | N.D. | ||||
| Mouse 3 | 37,648 | vs. M1: 0.98 | N.D. | N.D. | ||||
| Splenic B | IgH | 2 μg | JH2 | Mouse 1 | 66,589 | vs. M2: 0.98 | N.D. | N.D. |
| Mouse 2 | 81,547 | vs. M3: 0.98 | N.D. | N.D. | ||||
| Mouse 3 | 74,857 | vs. M1: 0.99 | N.D. | N.D. | ||||
| Splenic B | IgH | 2 μg | JH3 | Mouse 1 | 26,586 | vs. M2: 0.99 | N.D. | N.D. |
| Mouse 2 | 29,619 | vs. M3: 0.98 | N.D. | N.D. | ||||
| Mouse 3 | 23,097 | vs. M1: 0.99 | N.D. | N.D. | ||||
| Splenic B | IgH | 2 μg | JH4 | Mouse 1 | 21,532 | vs. M2: 0.97 | N.D. | N.D. |
| Mouse 2 | 26,838 | vs. M3: 0.95 | N.D. | N.D. | ||||
| Mouse 3 | 12,669 | vs. M1: 0.95 | N.D. | N.D. | ||||
| Splenic B | IgH | 2 μg | JH4 | Repeat 1 | 20,703 | vs. R2: 0.99 | 14,461 | vs. total: 1.00 |
| Repeat 2 | 18,959 | vs. R3: 0.99 | 13,045 | vs. total: 1.00 | ||||
| Repeat 3 | 22,118 | vs. R1: 0.99 | 14,799 | vs. total: 1.00 | ||||
| Splenic B | IgH | 500 ng | JH4 | Repeat 1 | 20,605 | vs. R2: 0.98 | 5,093 | vs. total: 0.99 |
| Repeat 2 | 18,897 | vs. R3: 0.97 | 5,374 | vs. total: 0.99 | ||||
| Repeat 3 | 20,105 | vs. R1: 0.97 | 5,570 | vs. total: 0.99 | ||||
| Splenic B | IgH | 100 ng | JH4 | Repeat 1 | 12,007 | vs. R2: 0.77 | 1,163 | vs. total: 0.96 |
| Repeat 2 | 6,968 | vs. R3: 0.79 | 649 | vs. total: 0.92 | ||||
| Repeat 3 | 8,106 | vs. R1: 0.86 | 896 | vs. total: 0.96 | ||||
| Splenic B | Igκ | 1 μg | Jκ1 | Mouse 1 | 46,554 | vs. C1: 0.99 | N.D. | N.D. |
| Jκ2 | Mouse 1 | 26,117 | vs. C1: 0.98 | N.D. | N.D. | |||
| Jκ4 | Mouse 1 | 16,047 | vs. C1: 0.98 | N.D. | N.D. | |||
| Jκ5 | Mouse 1 | 9,782 | vs. C1: 0.98 | N.D. | N.D. | |||
| Splenic B | Igκ | 1 μg | Jκ1 | Combined | 10,988 | vs. M1: 0.99 | N.D. | N.D. |
| Jκ2 | Combined | 10,159 | vs. M1: 0.98 | N.D. | N.D. | |||
| Jκ4 | Combined | 8,613 | vs. M1: 0.98 | N.D. | N.D. | |||
| Jκ5 | Combined | 17,750 | vs. M1: 0.98 | N.D. | N.D. |
N.D., not determined.
*
Mouse 1, 2, 3, the experiments were performed from three different mice; Repeat 1, 2, 3, the experiments were performed using DNA from the same mouse.
†
The correlation coefficient values (r) were derived from two sets of productive V(D)J exons (%) via the CORREL function in Excel. The bigger the value, the more similar the two sets of data.