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. 2016 Jun 20;113(28):7864–7869. doi: 10.1073/pnas.1522730113

Fig. S4.

Fig. S4.

Reactivity of rOCBs to antigen-transfected and nontransfected ethanol-fixed HEK293EBNA cells. We show triple-staining for FAM84A (A), AKAP17A (B), or MKNK1 (C) by rOCB antibodies (green; Left) and commercial antibodies (red; Center). Right shows the merged images. Nuclei are stained by DAPI. We compare HEK293EBNA cells that were transfected with the respective antigens (Upper) to untransfected HEK293EBNA cells (Lower). We used the following antibodies: rOCB-MS1-s2 and PA5-30705 for FAM84A-staining (A), rOCB-MS1-s8 and MBS711914 for AKAP17A staining (B), and scFv-MS2-s5 and Ab56425 for MKNK1 staining (C). All antibodies, except scFv-MS2-s5, stained antigen-transfected cells, but not untransfected cells, indicating that the endogenous expression levels are too low. (Scale bar: 50 µm.)