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. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Bio Protoc. 2015 Aug 20;5(16):e1567. doi: 10.21769/bioprotoc.1567

Figure 1. Blood-borne precursors contribute to macrophage populations in developing PyMT tumors.

Figure 1

Representative data expected from a parabiosis experiment. Two weight-matched, 8-week-old, congenically-marked female PyMT mice were surgically connected and maintained for approximately 3 months. The mammary tissue/tumors were removed from the unconnected side of each partner and pooled (tissue/tumors were pooled within each mouse, but not between mice). Tumors were processed as described above. The gated populations are defined as follows: CD45+MHCII+CD11bhi, mammary tissue macrophage (MTMs), CD45+MHCII+CD11blo, tumor-associated macrophages (TAMs), CD45+MHCII−CD11bhiLy6C+Ly6G+, neutrophils, and CD45+MHCII−CD11bhiLy6C+Ly6G−, monocytes.