Figure 4.

Sequence alignment of the heavy chain of the wild‐type (wt3H6), the superhumanized (su3H6) and the simulated (predictive, BM01–BM11) variants. The CDR regions as defined by Kabat were conserved during the humanization process and kept constant in all variants (but BM10) throughout this study (blue background). Identical amino acids compared to the murine template sequence are indicated as dots. Vernier zone residues of the heavy chain defined by Foote and Winter are marked by green frames. No light chain mutations in respect to the superhumanized variant were applied to the backmutation variants.