Fig. 7.

Nematostella vectensis α-catenin, but not a N. vectensis α·β-catenin heterodimer, inhibits Arp2/3 complex-mediated nucleation of F-actin. (A and B) Effect of N. vectensis α-catenin (A) or N. vectensis α·β-catenin heterodimer (B) on Arp2/3-mediated actin polymerization in a pyrene-actin assay. Reactions contained 2.32 μM of a 10% pyrene-actin mix (− control, black circles) plus 12.5 nM Arp2/3 complex and 23.2 nM WASp-VCA (+ control, grey diamonds), and the indicated concentrations of protein. Examples shown are each representative of at least three independent experiments.