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. 2016 Jul 18;11(7):e0159442. doi: 10.1371/journal.pone.0159442

Fig 3. Repeated closed head injury (CHI) induced chronic axonal degeneration in the optic tract and optic nerve.

Fig 3

Argyrophilic axons in the optic tract (A-F) were labeled using silver stain. Scale bars: 500μm A-C: 250μm D-F. The mean density of silver staining in the optic nerve was normalized to background using adjacent stained areas (G). * indicates p<0.05 compared to all other groups. Optic nerves (H-J) were labeled for neurofilament heavy chain (NF200) and the percent area of staining was quantified (K). Scale bars: 250μm H-J. $ indicates p<0.05 compared to sham.