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. 2016 Jul 18;11(7):e0159442. doi: 10.1371/journal.pone.0159442

Fig 4. Inflammation did not persist in the entorhinal cortex or hippocampus 10wks after repeated closed head injury (CHI).

Fig 4

Immunohistochemical labeling of ionized calcium-binding adaptor protein-1 (Iba-1; A-C) and glial fibrillary acidic protein (GFAP; D-I) in the entorhinal cortex and hippocampus after repeated sham (rSHAM), repeated CHI at 24h intervals (rCHI-24h), and repeated CHI at 48h intervals (rCHI-48h). Scale bars: 500μm A-F; 250μm G-I. The percent area of Iba-1 labeling was quantified for analysis in the entorhinal cortex (J). The mean integrated optical density (IOD) of GFAP/astrocyte was analyzed for the entorhinal cortex (K) and the hippocampus (L).