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. 2016 Jul 18;11(7):e0159508. doi: 10.1371/journal.pone.0159508

Fig 5. M8 variants with Asp at the CK2 and MAPK sites perturb R8 birth.

Fig 5

Eye discs were stained with α-Sens and α-ELAV to label differentiated R8s and secondary photoreceptors, respectively. Arrows indicate direction of MF, dotted circles denote missing R8s and secondary photoreceptors, and arrowheads denote recruitment defective R8s. (A) Discs expressing the CK2-mimic M8-S159D show sporadic gaps in Sens+ELAV clusters, whereas discs expressing the MAPK-refractory variant M8-S151A+S159D (D) closely resemble 109-68Gal4/+ (E) or WT disc (data not shown). Both, the MAPK mimic M8-S151D (B) and dual kinase (CK2+MAPK) mimic M8-S151D+S159D (C) exhibit areas lacking Sens+ELAV clusters with the greatest severity. (A’-D’) magnified view of panels A-D, and (A”-D”) Sens channel in greyscale.