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. 2016 Jul 18;11(7):e0159508. doi: 10.1371/journal.pone.0159508

Fig 9. MAPK site in M8 may be a target for the phosphatase PP2A.

Fig 9

(A) Co-expression of widerborst (wdb) rescues the reduced eye of the CK2 mimic M8-S159D, whereas that of LacZ elicits no rescue, as previously described [50]. Ommatidial (facet) counts were determined in 20 flies of the indicated genotypes. Values for genotypes upon co-expression of Wdb or LacZ were compared to the corresponding control (scaGal4/+; UAS-M8-S159D/+) flies (** denotes P-value < 0.001). In contrast, co-expression of Wdb does not rescue the reduced eye of the MAPK mimic M8-S151D (B) or the dual kinase (CK2+MAPK) mimic M8-S151D+S159D (C). (D) Quantitative analysis of eyes in panels B and C. For each genotype, the number of images analyzed for eye size (ommatidial/facet counts) is indicated. Genotypes shown in panels B and C were compared to their corresponding controls, i.e., values from Fig 4B and 4C, respectively (‘n.s.’ denotes not significant). Note that grey bar in panel D shows that the Gal4 driver alone or in combination with UAS-Wdb (shown in yellow inset above graph) has a wild type eye (>750 facets with normal hexagonal architecture) and was not used as a control for statistical comparisons.