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Specific binding to proline rich domain of tau confirmed for Tau-FL and tau22 fragment in samples 24h post-injury using (A) Tau1 and (B) Tau5 antibodies against total tau and compared to positive control transgenic AD model mouse brain lysate (3XTg-AD). The 22 kDa tau fragment (*) was detected by both total tau antibodies. Experiment replicated 3 times for (sham, PBBI).
