Fig 1. Mouse model to investigate the abscopal effect.

a. Schematic representation of the 67NR mammary carcinoma Balb/C mouse model and the timeline of treatments. 1 *10⁵ 67NR tumour cells were inoculated in the right (primary, straight line) and left (secondary, dashed line) flank on day 0 and 2, respectively. At day 14, animals were randomised based on the primary tumour size and assigned to four groups (untreated, Flt3-L, RT, RT+Flt3-L). Fractionated radiotherapy (RT, 3 x 8 Gy) was only given to the primary tumour at day 18, 19, and 20. Immunotherapy (IT, DC growth factor Flt3-L) was administered for ten consecutive days, started at day 19. The endpoint was at day 162 or a total tumour volume of 2cm³. b. Schematic representation of the irradiation setup. Four tumour-bearing mice (per session) were positioned in a customized Plexiglas tray. The animals’ body was protected by lead shielding except for the primary tumour that was placed under a linear accelerator. The star (*) in the top view indicates the position of the cross section view.