Fig 7. Evaluation of interactions between the LRR and NB(-ARC) domains of RPP1_NdA.

The association between the NB(-ARC1) subdomain and the LRR is not disrupted by the presence of ATR1 (A) or by mutations at the putative oligomerization interface (B). Constructs were transiently expressed in Nicotiana benthamiana and samples were collected at 48 hours post-infiltration (E = ATR1_Emoy2, C = ATR1_Cala2). Co-immunoprecipitations were performed using α-Flag agarose beads. The expression of ATR1:citrine was detected with an α-GFP antibody. Asterisks indicate non-specific bands. Staining of RuBisCO with Ponceau S provides a loading control. Experiments were performed three times with similar results.