Fig 8. Non-autoactive N-TIR domain mutants associate with the LRR domain of RPP1_NdA.
Constructs were transiently expressed in Nicotiana benthamiana and samples were collected at 36 hours post-infiltration. Co-immunoprecipitations were performed using α-Flag agarose beads. Staining of RuBisCO with Ponceau S provides a loading control. Experiments were performed three times with similar results.