Fig 1. Antibody-mediated inhibition of Pf RBC invasion.

RBC invasion inhibition efficiency, mediated by the human patients sera, was defined as the ratio of the subtraction of parasitemia in the test well (test patients sera) from the parasitemia in the control well (normal human serum) to the parasitemia in the control well, expressed as a percentage. 6Ab +ve refers to sera (both cured and recrudescent sera) containing specific antibodies against all six antigens (n = 14); Cured 5Ab +ve refers to cured sera containing specific antibodies against the five antigens (n = 14); 6Ab–ve refers to sera (both cured and recrudescent sera) with absence of specific antibodies against the six antigens (n = 6); Recrudescent 5Ab +ve refers to recrudescent sera containing specific antibodies against the five antigens (n = 7). Pooled cured refers to sera pooled from all cured patients (n = 40) and pooled recrudescent refers to sera pooled from all recrudescent patients (n = 28). Each point in graph represents one P. falciparum isolate (n = 12). Horizontal line in graph represents mean inhibition efficiency between the 12 P. falciparum isolates. * denotes p <0.05; ** denotes p <0.01; *** denotes p <0.001; **** denotes p <0.0001, by Mann-Whitney U-test (adjusted for multiple comparison using Bonferroni correction).