. 2016 Jul 18;10(7):e0004847. doi: 10.1371/journal.pntd.0004847

Table 1. Characteristics of the Burkholderia pseudomallei antigens used in this study.

	Locus Bps^a	Used name for protein array	Protein name^a	Definition^a	Function^a	Genome location^b	Expressed amino acids^c	Tag^d	Protein location^e
1	BPSL0280	BPSL0280	FlgK	flagellar hook-associated protein FlgK	cell motility	Chr. 1	total (aa 667)	Strep	e
2	BPSL1445	BPSL1445	-	putative lipoprotein	unknown	Chr. 1	aa 23 to 195	Strep	e/m
3	BPSL1661	BPSL1661-1001	-	putative hemolysin-related protein	unknown	Chr. 1	aa 1 to 683	Strep	e
4	BPSL1661	BPSL1661-1002	-	putative hemolysin-related protein	unknown	Chr. 1	aa 1001 to 2000	Strep	e
5	BPSL2030	BPSl2030	-	putative exported protein	unknown	Chr. 1	aa 23 to 186	Strep	e
6	BPSL2096	BPSL2096	-	hydroperoxide reductase	detoxification	Chr. 1	total (aa 182)	Strep/His	c
7	BPSL2520	BPSL2520	-	putative exported protein	unknown	Chr. 1	aa 22 to 198	Strep	e
8	BPSL2522	BPSL2522	-	outer membrane protein a precursor	unknown	Chr. 1	aa 23 to 224	Strep	om
9	BPSL2697	BPSL2697	GroEL	60 kDa molecular chaperone GroEL	protein folding and stabilisation	Chr. 1	total (aa 546)	Strep/His	c
10	BPSL2698	BPSL2698	GroES	10 kDa molecular chaperone GroES	protein folding and stabilisation	Chr. 1	total (aa 97)	Strep	c
11	BPSL3319	BPSL3319	FliC	flagellin	cell motility	Chr. 1	total (aa 388)	Strep	e
12	BPSS0476	BPSS0476	GroES	10 kDa chaperonin	protein folding and stabilisation	Chr. 2	total (aa 96)	Strep	c
13	BPSS0477	BPSS0477	GroEL	60 kDa chaperonin	protein folding and stabilisation	Chr. 2	total (aa 546)	Strep/His	c
14	BPSS0530	BPSS0530	-	conserved hypothetical protein	protein secretion, Type VI system	Chr. 2	total (aa 453)	Strep	c
15	BPSS1385	BPSS1385	-	ATP/GTP binding protein	unknown	Chr. 2	total (aa 328)	Strep	c
16	BPSS1516	BPSS1516	BopC	effector protein[28]	virulence, effector protein	Chr. 2	total (aa 469)	Strep	c
17	BPSS1525	BPSS1525-79	BopE	G-nucleotide exchange factor	virulence, effector protein	Chr. 2	aa 79 to 261	Strep	e
18	BPSS1532	BPSS1532-344	BipB	putative cell invasion protein	virulence, effector protein	Chr. 2	aa 1 to 344	Strep/His	e
19	BPSS1722	BPSS1722	Mdh	malate dehydrogenase	citrate cycle	Chr. 2	total (aa 327)	Strep	c
20	BPSS2141	BPSS2141	OppA	periplasmic oligopeptide-binding protein precursor	transport	Chr. 2	aa 40 to 554	Strep	p

^a Locus name, protein name, definition and function were used from B. pseudomallei strain K96243 and obtained from Kyoto Encyclopedia of Genes and Genomes (KEGG) (www.genome.jp/kegg/)

^b Location of the respective gene in the genome of B. pseudomallei K96243. Chr. 1 = chromosome 1; Chr.2 = chromosome 2

^c Shows length and/or expressed part of the respective protein. Proteins were expressed without signal sequences and membrane domains. aa—amino acid; total—whole protein

^d Tag indicates the used C-terminal amino acid sequence used for purification. Strep–Strep-tag; His–His-tag

^e Protein location was determined by PSORTb 3.0.2 (Prediction of Protein Sorting Signals and Location Sites in Amino Acid Sequences) http://www.psort.org/psortb/

c–cytoplasm, e–extracellular, m–membrane, om–outer membrane, p—periplasm