Fig 6. Replication characteristics of chromosomes in ΔparAc1 mutant cells.

A. DNA purified from exponentially-growing FBP47 (Nel13 ΔparAc1) cells was purified, processed and analyzed in parallel to wild type (Nel13) DNA, as outlined in the Fig 2 legend. The FBP47 data are shown in orange superimposed on the Nel13 data in blue (transposed from Fig 2). Raw read numbers are shown for c1 and c2: these reflect relative quantities of loci within a given strain but not of a given replicon between wt and mutant strains. B. Positions of c1 and c2 origins in wild type and ΔAc1 mutant cells growing in MglyC medium at 30°C. Pole-to-focus distances are shown black for one-focus cells and coloured for two-focus cells, as in Fig 3. In these conditions, cells grow more slowly (τ ~140 mins) than in the previous analyses of focus position shown in Figs 3 and 4 (τ ~110 mins), and the cells are correspondingly smaller.