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. 2016 Jul 18;11(7):e0159034. doi: 10.1371/journal.pone.0159034

Fig 6. Cellular proteasomal activity was inhibited by arenobufagin, which might be related to the binding of Na, K-ATPase.

Fig 6

(A) Inhibition of arenobufagin (Arg) at different doses on cytosolic proteasomal activity for 24h. (B) Three types of cellular proteasome enzyme activities (C-L, T-L and CT-L) in HeLa cells treated with 0.1% DMSO or arenobufagin(Arg) at different concentrations for 24h. (C) Western blotting results of WEE1 and actin in control HeLa cells and cells treated with 10 nM arenobufagin for 24 h and 48 h. Each blot was the representative result of three independent experiments. (D) Inhibition of antibodies against α1 or α3 subunits of Na,K-ATPase or combination of antibodies against α1 or α3 subunits of Na,K-ATPase and 10 nM arenobufagin on cytosolic proteasomal activity for 24h,48h and 72h, respectively. Data were statistical results of three independent experiments. Data were statistical results of three independent experiments. Data were expressed as mean ± SD. *Significant difference from the control group at P<0.05.