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. 2016 Jun 30;49(3):887–894. doi: 10.3892/ijo.2016.3593

Figure 2.

Figure 2

The expression of PLXND1 and SEMA3E in human gastric cancer cell lines, and activation of Erk by SEMA3E in human gastric cancer cell lines. The generation of SEMA3E up- and downregulated gastric cancer cells. (A) PLXND1 and SEMA3E expression levels of several gastric cancer cell lines were examined by qRT-PCR and normalized by the GAPDH expression level. GCIY expressed high levels of PLXND1 and SEMA3E. (B) SEMA3E phosphorylated Erk in MKN74 cells, which endogenously express very low levels of PLXND1. Moreover, MKN74-SEMA3E-HA cells phosphorylated Erk more than MKN74-EV. (C) Two types of stably shSEMA3E-expressing GCIY and HGC-27 cells were generated and the qRT-PCR revealed that the SEMA3E expression levels were effectively reduced in these knockdown cells. (D) Stably SEMA3E-overexpressing MKN74 cells (MKN74-SEMA3E-HA) were generated and the qRT-PCR and western blot analysis revealed that the SEMA3E expression levels were effectively increased in MKN-SEMA3E-HA.