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. 2016 Apr 12;16(5):3042–3050. doi: 10.1021/acs.nanolett.6b00054

Figure 5.

Figure 5

Differentiation of ST2 bone marrow stromal cells treated with the LRAP signaling molecule to activate the Wnt pathway and weak β-sheet PA assemblies. Real time quantitative RT-PCR analysis of osteoblast cell marker gene mRNA expression and mineral deposition by ST2 cells. (A) Two days after osteo-induction, RNA was isolated for qRT-PCR analysis of osteoblast marker genes: Runx2, Osx, Dlx5, and type I collagen (coll. I). Data is expressed as a relative fold increase in gene expression of conditions containing weak β-sheet PA assemblies normalized to conditions with LRAP but lacking PA assemblies. ***p < 0.001, **p < 0.01, nonparametric t-test comparing to conditions with the same LRAP concentration but lacking PA nanofibers (n = 3). (B) Two weeks after bone induction, mineral deposition was assayed with Alizarin Red staining and (C) quantified. *p = 0.018, nonparametric t-test comparing conditions with the same concentration of LRAP but lacking PA nanofibers (n = 3). Data is represented as a relative increase in mineral deposition for cells treated with the weak β-sheet PA nanofibers normalized to the LRAP only treatment baseline. OM: Osteogenic media without LRAP.