Skip to main content
. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Mol Cancer Ther. 2008 Dec;7(12):3825–3833. doi: 10.1158/1535-7163.MCT-08-0730

Figure 1.

Figure 1

Expression of Nurr1 and Nur77 protein in urothelial carcinoma cells and structure-dependent Nurr1 activation by C-DIM. A, Nurr1 and Nur77 expression in bladder cancer cells. Whole-cell lysates from 253J B-V, 253J P, KU7, UM-UC3, UM-UC5, UM-UC6, UM-UC9, UM-UC10, UM-UC13, and UM-UC14 cells were analyzed by Western blot analysis as described in Materials and Methods. B to D, activation of GAL4-Nurr1/pGAL4, GAL4-Nor1/pGAL4, GAL4-Nur77/pGAL4, NuRE, PM-GAL4/pGAL4, or RXR-GAL4/pGAL4 by C-DIM compounds. KU7 bladder cancer cells were transfected with GAL4-Nurr1/pGAL4, GAL4-Nor1/pGAL4, GAL4-Nur77/pGAL4, NuRE, PM-GAL4/pGAL4, or RXR-GAL4/pGAL4 and treated with DMSO or different concentrations of the C-DIMs and luciferase. Columns, mean of at least three replicate experiments for each treatment group; bars, SE. *, P < 0.05.