Skip to main content
. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Mol Cancer Ther. 2008 Dec;7(12):3825–3833. doi: 10.1158/1535-7163.MCT-08-0730

Figure 3.

Figure 3

Effects of DIM-C-pPhCl on apoptosis. A, dose-dependent induction of apoptosis in KU7 cells by DIM-C-pPhCl. KU7 cells were treated with different concentrations of DIM-C-pPhCl for 48 h, and whole-cell lysates were analyzed by Western blot analysis. B, time-dependent induction of apoptosis by DIM-C-pPhCl in 253J B-V cells. 253J B-V cells were treated with 5 μmol/L C-DIM-pPhCl for 24 and 48 h, and whole-cell lysates were analyzed by Western blot analysis. C, effects of z-VAD-fmk in PARP cleavage. 253J B-V cells were treated with DIM-C-pPhCl alone or in combination with 10 μmol/L pan-caspase inhibitor z-VAD-fmk, which was administrated 1 h before the addition of 5 μmol/L DIM-C-pPhCl. After 48 h, whole-cell lysates were analyzed for cleaved PARP and TRAIL induction by Western blot analysis as described in Materials and Methods. D, induction of TRAIL mRNA by DIM-C-pPhCl in T24 cells. cDNAs were generated from 1 μg total RNAs from bladder cancer cells with or without 5 μmol/L DIM-C-pPhCl. Reverse transcriptase reaction was carried as described in Materials and Methods.