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. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Mol Cancer Ther. 2008 Dec;7(12):3825–3833. doi: 10.1158/1535-7163.MCT-08-0730

Figure 4.

Figure 4

Nurr1-dependent induction of TRAIL and cleaved PARP in 253J B-V cells. Cells were transfected with nonspecific control (iNS) or iNurr1 and treated with DMSO or 10 μmol/L DIM-C-pPhCl, and whole-cell lysates were analyzed by Western blot analysis for Nurr1, PARP cleavage, TRAIL, and actin (loading control) proteins (A). Columns, mean of three replicate experiments for each treatment group; bars, SE. Protein levels were normalized to actin. Significant (P < 0.05) inhibition of Nurr1 expression (B), decreased expression induction of PARP cleavage (C), and TRAIL (D) by iNurr1 are quantitated and 100% protein expression was assigned to levels in cells treated with DIM-C-pPhCl and transfected with nonspecific control. Each experiment was reproduced three times independently.