Nurr1-dependent induction of TRAIL and cleaved PARP in 253J B-V cells. Cells were transfected with nonspecific control (iNS) or iNurr1 and treated with DMSO or 10 μmol/L DIM-C-pPhCl, and whole-cell lysates were analyzed by Western blot analysis for Nurr1, PARP cleavage, TRAIL, and actin (loading control) proteins (A). Columns, mean of three replicate experiments for each treatment group; bars, SE. Protein levels were normalized to actin. Significant (P < 0.05) inhibition of Nurr1 expression (B), decreased expression induction of PARP cleavage (C), and TRAIL (D) by iNurr1 are quantitated and 100% protein expression was assigned to levels in cells treated with DIM-C-pPhCl and transfected with nonspecific control. Each experiment was reproduced three times independently.