Table 1.

Effect of various inhibitors and stimulators on the ¹²⁵I-sCD14 proteolysis by Prevotella intermedia protease. TLCK Tosyl-L-lysine chloromethyl ketone, TPCK tosyl-L-phenylalanine chloromethyl ketone, DFP diisopropylfluorophosphate, PMSF phenylmethylsulfonyl fluoride, EDTA ethylenediamine tetraacetic acid(pH 7.0), EGTA ethyleneglycol-bis-N,N,N′,N′ tetraacetic acid

Reagent	Class	Concentration	Activity (%)
MEM	–	–	100
N-ethylmaleimide	Cysteine	5 mM	<14
Iodoacetamide	Cysteine	5 mM	<7
TLCK	Serine/cysteine	0.5 mM	<5
TPCK	Serine/cysteine	0.5 mM	84
TPCK	Serine/cysteine	1 mM	32
TPCK	Serine/cysteine	2 mM	<10
Leupeptine	Serine/cysteine	1 mM	<2
Antipain	Serine/cysteine	1 mM	<3
DFP	Serine	0.5 mM	96
PMSF	Serine	5 mM	100
Dichloroisocoumarin	Serine	0.5 M	100
L-Cysteine	-	10 mM	146
L-Cysteine	-	30 mM	206
Dithiothreitol	Reducing agent	15 mM	154
Dithiothreitol	Reducing agent	30 mM	212
EDTA	Metallo	10 mM	72
EDTA	Metallo	50 mM	<5
EGTA	Metallo	10 mM	<9
Phosphoramidon	Metallo	0.2 mM	<12
Poly-L-arginine	Arginine	1 mg/ml	<35