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Effect of 6% TENS on IL-1β-induced inhibition of osteocalcin and alkaline phosphatase synthesis. (A) RT-PCR analysis of mRNA for osteocalcin in PDL cells exposed to various treatments regimens for 4, 24, or 48 h. Densitometric analysis of western blots showing osteocalcin synthesis (B), and alkaline phosphatase activity (C), in cells subjected to indicated treatment regimens for 24 or 48 h. Data represent mean and SEM of triplicate values. *p < 0.05 vs. cells treated with IL-1β alone.
