Figure 3.
Inhibition of rHuTNFα-dependent cyclooxygenase 2 (COX-2) mRNA expression and prostaglandin E2 (PGE2) synthesis by CTS in articular chondrocytes. A, COX-2 mRNA expression in chondrocytes either untreated or subjected to rHuTNFα (1 ng/ml), CTS alone, or rHuTNFα and CTS for 4, 24, or 48 hours. B, PGE2 synthesis in chondrocytes subjected to treatment regimens described in A for 8, 24, or 48 hours, as measured in the culture supernatants by radioimmunoassay. Data in A represent 1 of 3 separate experiments. Data in B represent means and SEM of triplicate values. * = P ≤ 0.05 versus cells treated with rHuTNFα alone. See Figure 1 for other definitions.