Fig. 1.

Ubiquitin protein Ub3881 expression is decreased during DENV infection in Ae. aegypti. A. Ae. .aegypti were infected with DENV and microarray analysis for gene expression was done at 1, 2 and 7 days post-infection on isolated RNA, known ubiquitin family genes are shown. P < 0.05. Pooled mosquitoes. Experimental and technical replicates were done in triplicate. B. Alignment using ClustalOmega of the first active length of the ubiquitin genes from 1A. C/D. Mosquitoes were infected with DENV via blood feeding and at 1, 2 and 7 days post-infection, C. RNA was isolated from whole mosquitoes or D. RNA was isolated from dissected midguts and salivary glands. qRT-PCR analysis was done to measure expression of ubiquitin genes. Experimental and technical replicates were done in triplicate. P < 0.05. Statistical analysis done using ANOVA and Students t-tests. Fold-change in expression is shown as compared to uninfected, bloodfed mosquitoes.