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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Crit Care Med. 2016 Aug;44(8):e651–e663. doi: 10.1097/CCM.0000000000001637

Figure 11.

Figure 11

SRT1720 activates the mitochondrial biogenesis pathway in primary hepatocytes after H/R. Primary hepatocytes underwent oxyrase-induced hypoxia in glucose- and pyruvate-free media followed by reoxygenation in complete media. Cells were lysed and mRNA and protein were used for analysis. Primary hepatocytes were subjected to 2 h of hypoxia followed by 1 h (A) or 4 h of reoxygenation (B). Cells were treated with 250 nM (L) or 1000 nM (H) of SRT1720 at reoxygenation, * P < 0.05 versus normoxia (N). (A) mRNA expression of the mitochondrial biogenesis marker nuclear transcription factor-1 (Nrf-1) and (B) protein expression of mitochondrial transcription factor A (mtTFA) were measured by RT-PCR and western blot, respectively. * P < 0.05 versus Normoxia (N). Data are presented as means ± SE (n = 3/group), representative of 2 independent experiments.