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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Crit Care Med. 2016 Aug;44(8):e651–e663. doi: 10.1097/CCM.0000000000001637

Figure 8.

Figure 8

SRT1720 enhances autophagy in H4IIE hepatocytes after hypoxia-reoxygenation (H/R). H4IIE hepatocytes underwent 6 h of oxyrase-induced hypoxia in glucose-, pyruvate- and serum-free media followed by 4 h or 24 h of reoxygenation in complete media. At the beginning of reoxygenation, cells were treated with 0.125% DMSO (vehicle) or 500 nM of SRT1720. (A) Western blotting against microtubule associated protein 1 light chain 3 (LC3) in total cell lysate. (B) Representative images of H4IIe cells (green) stained with acridine orange at 24 h of reoxygenation. White arrows point to autophagic vesicles (red). Data representative of three independent experiments.