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Immunoblot of membrane protein isolated from the surgical shams or 8min CA mice treated with either NS or 7.5% HS for 24 hr. Naïve WT mice were used as controls. AQP4 was identified as a major band at ∼ 30 kDa. Densitometric analysis (intensity ratio) for the AQP4 protein was corrected to a standard protein (β–actin).
