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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Ann Rheum Dis. 2016 Jan 18;75(11):2007–2013. doi: 10.1136/annrheumdis-2015-208441

Figure 3. Decreased SMG7 levels associated with increased ANA.

Figure 3

(A) SMG7 mRNA levels in PBMCs correlated inversely with ANA titers of SLE patients. Among 68 patients, 56 were recruited at UCLA and 12 were recruited at OMRF, and their ANA titers were measured in Clinical Laboratory at each site. (B&C) Increased ANA IgG and CCL19 levels after SMG7 silencing in SLE PBMCs. PBMCs from ANA positive SLE patients (n=13) were incubated for 5 days in the presence or absence of siRNA targeting SMG7, GAPDH or siRNA with a non-targeting sequence (NC), respectively. ANA IgG (B) and CCL19 (C) levels in culture supernatants were measured by ELISA, and plotted as fold change with respect to mock (culture medium only). Results are presented as mean ± SEM.