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. 2016 Jul 19;3:32. doi: 10.3389/fmolb.2016.00032

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Copyright © 2016 Gruber, Lang, Rajendra, Nuk, Raffl, Schildbach and Zechner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ParM binds ATPases TraI, TraD, and TraC in vivo. E. coli MS411 cells carrying plasmids with the indicated tra-genes (above) and coexpressing FLAG-tagged or wild type par- alleles (left) were lysed and treated with formaldehyde to cross-link interacting proteins. Co-retention of Par protein-binding partners on FLAG-affinity matrix was monitored by Western analysis. Antibodies to the transfer proteins indicated were used for detection in whole cell lysates (WCL) and in the fractions retained on FLAG affinity beads (IP).