Table 3.
Oligos used in this study.
| Name | 5′-3′ Sequence |
|---|---|
| CLONING | |
| parMRCFW | GACGAATTCCACTTTTGTTACCCGCC |
| parCrev | GACGAATTCTTAATTTATAAAACTCCTTATGG |
| parM NdeI FW | TTTTCATATGTTGGTATTCATTGATGACG |
| parM BamHI REV | TTTTGGATCCTTAATTACCTATGAGATACATACC |
| ParM_SFW | ATAGTAGGTACCTTGGTATTCATTGATGACG |
| ParM_SRev | GCAATCGTCGACTTAATTACCTATGAGATACATACCGT |
| ParR_FW | ATAGTAGGTACC-ATGGACAAGCGCAGAACC |
| ParR_Rev | GCAATCGTCGACTTATTAATTTATTAGCTTCATCGC |
| parR_NheIFW | GTAGCTAGCATGATGGACAAGCGCAGAACC |
| parR_NheIRev | GTAGCTAGCATTTATTAGCTTCATCGC |
| parMBamHIfw | TTTTGGATCCATGTTGGTATTCATTGATGACG |
| parMEcoRIrev | TTTTGAATTCTTAATTACCTATGAGATACATACC |
| parRBamHIfw | TTTGGATCCATGATGGACAAGCGCAGAAC |
| parREcoRIrev | TTTGAATTCTTAATTTATTAGCTTCATCG |
| ParMloxFW | CCCAAAACATACCCAAACACACACCAAAAAAACACCATAAGGAGTTTTATAatataacttcgtataG |
| ParMloxRev | GTTTGATTTACATCTGGATTTAGTTTGAAGGCAATGGTTCTGCGCTTGTCCATCAGGataacttcgtataA |
| FW_parR_TetRA | AACCAATAACTCTCAATATGATTTAGTTAACGGTATGTATCTCATAGGTAATTACAAGAATTGCCGGCGGAT |
| Rev_parR_TetRA | GTTCCCTTTATCCAGCCTGATAGTGGATAAAGGGAACTCAATAATAATTGAAGGTATTTCACACCGCATAGC |
| SS01 | GCCGAATTCATGAGTTTTAACGCAAAG |
| SS02 | CGTGAAGCTTTCAGAAATCATCTCCCG |
| parM_CFLAG_EcoRI_rev | TTATAGAATTCTTACTTGTCATCGTCATCCTTGTAATCATTACCTATGAGATACATACC |
| parR_NFLAG_BamHI_fw | TATAGGATCCATGGATTACAAGGATGACGATGACAAGATGGACAAGCGCAGAAC |
| parR_CFLAG_EcoRI_rev | TTATAGAATTCTTACTTGTCATCGTCATCCTTGTAATCATTTATTAGCTTCATCGC |
| StrepTraCEcorIFw | AAGAATTCAATAACCCACTTGAGGCCG |
| StrepTraCHindIIIR | TTTAAGCTTTCATGCAACACTCCTGTATTT |
| EMSA AND FLUORESCENCE INTENSITY | |
| parC* | AAACAAAACCCAAAAACAACCC |
| parCcomp | GGGTTGTTTTTGGGTTTTGTTT |
| oriT* | ACCAAAAGCACCACACCCCACGCAAAAACAAG |
| oriTcomp | CTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGT |
| randomseq* | CGAACGAGCAGTTGTTTCAGCG |
| randomseqcomp | CGCTGAAACAACTGCTCGTTCG |
| oriT17* | TTTGCGTGGGGTGTGGT |
| 2 x G144C | TTTTGCGTGGGCTGTGGTCTTTGCGTGGGCTGTGGTCTTT |
*
= 3′-carboxytetramethyl-rhodamine (TAMRA) labeled, Restriction sites are marked in cursive, FLAG-sequences are underscored, lox-sites are in lower-case letters.