Table 3.
Name | 5′-3′ Sequence |
---|---|
CLONING | |
parMRCFW | GACGAATTCCACTTTTGTTACCCGCC |
parCrev | GACGAATTCTTAATTTATAAAACTCCTTATGG |
parM NdeI FW | TTTTCATATGTTGGTATTCATTGATGACG |
parM BamHI REV | TTTTGGATCCTTAATTACCTATGAGATACATACC |
ParM_SFW | ATAGTAGGTACCTTGGTATTCATTGATGACG |
ParM_SRev | GCAATCGTCGACTTAATTACCTATGAGATACATACCGT |
ParR_FW | ATAGTAGGTACC-ATGGACAAGCGCAGAACC |
ParR_Rev | GCAATCGTCGACTTATTAATTTATTAGCTTCATCGC |
parR_NheIFW | GTAGCTAGCATGATGGACAAGCGCAGAACC |
parR_NheIRev | GTAGCTAGCATTTATTAGCTTCATCGC |
parMBamHIfw | TTTTGGATCCATGTTGGTATTCATTGATGACG |
parMEcoRIrev | TTTTGAATTCTTAATTACCTATGAGATACATACC |
parRBamHIfw | TTTGGATCCATGATGGACAAGCGCAGAAC |
parREcoRIrev | TTTGAATTCTTAATTTATTAGCTTCATCG |
ParMloxFW | CCCAAAACATACCCAAACACACACCAAAAAAACACCATAAGGAGTTTTATAatataacttcgtataG |
ParMloxRev | GTTTGATTTACATCTGGATTTAGTTTGAAGGCAATGGTTCTGCGCTTGTCCATCAGGataacttcgtataA |
FW_parR_TetRA | AACCAATAACTCTCAATATGATTTAGTTAACGGTATGTATCTCATAGGTAATTACAAGAATTGCCGGCGGAT |
Rev_parR_TetRA | GTTCCCTTTATCCAGCCTGATAGTGGATAAAGGGAACTCAATAATAATTGAAGGTATTTCACACCGCATAGC |
SS01 | GCCGAATTCATGAGTTTTAACGCAAAG |
SS02 | CGTGAAGCTTTCAGAAATCATCTCCCG |
parM_CFLAG_EcoRI_rev | TTATAGAATTCTTACTTGTCATCGTCATCCTTGTAATCATTACCTATGAGATACATACC |
parR_NFLAG_BamHI_fw | TATAGGATCCATGGATTACAAGGATGACGATGACAAGATGGACAAGCGCAGAAC |
parR_CFLAG_EcoRI_rev | TTATAGAATTCTTACTTGTCATCGTCATCCTTGTAATCATTTATTAGCTTCATCGC |
StrepTraCEcorIFw | AAGAATTCAATAACCCACTTGAGGCCG |
StrepTraCHindIIIR | TTTAAGCTTTCATGCAACACTCCTGTATTT |
EMSA AND FLUORESCENCE INTENSITY | |
parC* | AAACAAAACCCAAAAACAACCC |
parCcomp | GGGTTGTTTTTGGGTTTTGTTT |
oriT* | ACCAAAAGCACCACACCCCACGCAAAAACAAG |
oriTcomp | CTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGT |
randomseq* | CGAACGAGCAGTTGTTTCAGCG |
randomseqcomp | CGCTGAAACAACTGCTCGTTCG |
oriT17* | TTTGCGTGGGGTGTGGT |
2 x G144C | TTTTGCGTGGGCTGTGGTCTTTGCGTGGGCTGTGGTCTTT |
= 3′-carboxytetramethyl-rhodamine (TAMRA) labeled, Restriction sites are marked in cursive, FLAG-sequences are underscored, lox-sites are in lower-case letters.