Table 5.
Chemical composition of total cell wall sugars in senesced mature dried stems from wild-type and pAtIRX5:AdoMetase (AdoMetase) lines.
| Sugar | Mean ± SE (mg g−1 dry weight) |
|||
|---|---|---|---|---|
| Wild type | AdoMetase-1 | AdoMetase-2 | AdoMetase-3 | |
| Fucose | 3.1 ± 0.1 | 3.1 ± 0.0 | 3.1 ± 0.0 | 3.1 ± 0.0 |
| Rhamnose | 7.2 ± 0.2 | 6.6 ± 0.1 | 6.5 ± 0.2 | 6.4 ± 0.2 |
| Arabinose | 7.8 ± 0.1 | 8.1 ± 0.3 | 7.6 ± 0.3 | 7.9 ± 0.2 |
| Galactose | 13.2 ± 0.4 | 13.5 ± 0.4 | 13.3 ± 0.4 | 13.7 ± 0.3 |
| Mannose | 22.7 ± 1.8 | 35.3 ± 3.2* | 30.8 ± 3.2* | 29.8 ± 0.9* |
| Galacturonic acid | 61.9 ± 8.9 | 51.9 ± 6.9 | 55.6 ± 5.3 | 65.8 ± 1.1 |
| Xylose | 185.1 ± 12.7 | 220.8 ± 9.9 | 192.9 ± 10.0 | 184.2 ± 9.4 |
| Glucose | 376.4 ± 9.2 | 433.3 ± 13.4* | 422.2 ± 15.7* | 413.7 ± 13.7* |
| Glucose (TFA) | 10.4 ± 0.3 | 12.9 ± 0.2* | 14.3 ± 0.3* | 14.2 ± 0.3* |
| Glucuronic acid | 2.2 ± 0.1 | 5.2 ± 0.8* | 5.2 ± 0.8* | 5.6 ± 0.2* |
| 4-O-Methylglucuronic acid | 6.8 ± 0.8 | 1.9 ± 0.3* | 1.9 ± 0.1* | 1.9 ± 0.2* |
“Glucose (TFA)” is the amount of glucose released after trifluoroacetic acid hydrolysis of cell wall residues. Values are means ± SE from three biological replicates (n = 3). Asterisks indicate significant differences from the wild type using the unpaired Student’s t-test (*P < 0.05).