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. 2016 Jul 1;30(13):1515–1528. doi: 10.1101/gad.283895.116

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© 2016 Dowen et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — The hypodermal heterochronic gene lin-29 modulates intestinal expression of the vit genes. (A) Images of GFP fluorescence for wild-type and lin-29(n333) adult animals carrying the Pvit-3::GFP transgene. (B) RT-qPCR analysis of vit gene expression after systemic, intestinal-specific, or hypodermal-specific knockdown of lin-29 by RNAi. Data are plotted relative to a control RNAi treatment and represent the mean ± SEM of three biological replicates. (C) Representative GFP fluorescence images of wild-type, lin-29(n333), and lin-29(n333) transgenic animals that express the indicated rescue constructs. All animals also carry the Pvit-3::GFP transgene.