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. 2016 Jul 1;30(13):1515–1528. doi: 10.1101/gad.283895.116

Figure 6.

Figure 6.

Targets of mTORC2 are required for maintaining fat content and body size. (A,B) Representative images (A) and quantification (B) of wild-type, lin-29(n333), pdk-1(sa680ts), akt-1(mg306), and sgk-1(ok538) adult animals stained with Oil Red O. Animals were grown at 15°C until they reached the L4 stage before shifting to 25°C for 24 h. (C) Quantification of Oil Red O staining for wild-type, pqm-1(ok485), sgk-1(ok538), and pqm-1(ok485); sgk-1(ok538) adult animals grown at 20°C. Two independent replicate experiments are shown. (D,E) Body size measurements were extracted from the corresponding Oil Red O images used in B and C (replicate 1). All Oil Red O quantification data and body size measurements are plotted as the mean and the 95% confidence interval. The P-values were generated by one-way ANOVA using a Bonferroni's multiple comparison test ([*] P < 0.0001) (B,D) or a two-tailed Student's t-test ([*] P < 0.0001; [#] P = 0.072; [##] P = 0.006) (C,E).