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. 2016 Jul 1;30(13):1542–1557. doi: 10.1101/gad.284166.116

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© 2016 Fan et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — The kinase activity of FER and its downstream effector, MET, were essential for cell motility. (A) MEF cell lysates Fer^+/+, Fer^DR/DR, and Fer^DR/DR rescued with either 6xMYC-tagged wild type or the kinase-dead FER-K592R mutant were immunoblotted with antibody against FER to demonstrate comparable expression. Actin was probed as the loading control. (B,C) A Boyden chamber assay was performed on the indicated MEF cells, in which migration was monitored 24 h after seeding. Representative images are illustrated (B), along with quantitation (mean ± SEM; n = 3) (C). (D) Both MET siRNAs and a FER expression construct were delivered as indicated into CAOV4 cells by electroporation. After 48 h, the expression levels of MET and FER were measured by immunoblotting, with actin as a loading control. (E,F) Forty-eight hours after electroporation and seeding, a wound healing assay was performed on the cell lines indicated in D. Wound recovery was recorded 6 h after scratch injury. Representative images are illustrated (E), together with quantitation (mean ± SEM; n = 3) (F).